From Wikipedia, the free encyclopedia. This article has multiple issues. The friendliest, high quality science and math community on the planet! Retrieved from ” https: Nov 24, 1. The Scatchard equation is an equation used in molecular biology for calculating the affinity constant of a ligand with a protein. This article should be divided into sections by topic, to make it more accessible. Log in or Sign up.
Relative binding affinities between two sites can be distinguished with a line showing identical affinity and a curve showing different affinities. Nov 24, 3. Thus any experimental error will be present in both axes. The intercept on the X axis is B max. For figs if you can scan them pics can be put up by loading them into tinypic. Their original intention was to transform the data into linear representations of the original data such that linear regression methods could be applied. No, create an account now. Nov 28, 8.
This page was last edited on 22 Octoberat It is named after the American chemist George Scatchard  and is sometimes referred to as the Rosenthal-Scatchard equation. Join Physics Forums Today! It is also more robust against error-prone data than the Lineweaver—Burk plotparticularly because it gives equal weight to data points in any range of substrate concentration or reaction rate. It turns out that the answer was 6.
This page was last edited on 17 Februaryat Retrieved from ” https: You can help Wikipedia by expanding it. Shouldn’t it be -3 so that Ks is 3? Their original intention was to transform the data into linear representations of the original data such that linear regression methods could be applied.
I also noticed this is my first post Now take uMol and divide it by 10uL; 10uL because that’s how much you extracted in the experiment. Therefore, the typical measure of goodness of fit for linear regressionthe correlation coefficient Ris not applicable.
A Scatchard plot is a plot of the ratio of concentrations of bound ligand to unbound ligand versus the bound ligand concentration. Do you already have an account? Biochemistry methods Proteins Biochemistry stubs. No, create an account now. Like other techniques that linearize the Michaelis—Menten equationthe Eadie-Hofstee plot was used historically for rapid identification of important kinetic terms like K M and V maxbut has been superseded by nonlinear regression methods that are significantly more accurate and no longer computationally inaccessible.
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Eadie–Hofstee diagram – Wikipedia
Any help would be greatly appreciated! Nov 24, 2. You just copy what it gives you and paste it here and it comes up – there used to be a delay here for checks but I think there isn’t any more.
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Nov 27, 5. These transformations frequently distort experimental error and can be misleading if results are not accurate.
Views Read Edit View history. Please help improve the article with a good introductory style. For figs if you can scan them pics can be put up by loading them into tinypic. Relative binding affinities between two sites can be distinguished with a line showing identical affinity and a curve showing different affinities.
Please help by adding section headings in accordance with Wikipedia’s Manual of Style. Thank you for clearing up the units for me. I checked my math and I got 6 binding sites too. Saturation Binding Curves and Scatchard Plots”.
Eadie Scatchard Plot | Physics Forums
Nov 28, 9. Retrieved from ” https: Both plots remain useful as a means to present data graphically. Nov 28, 7.
Nov 28, 8. Applications in the Life Sciences. The Scatchard equation is an equation used in molecular biology for calculating the affinity constant of a ligand with a protein.
One drawback from the Eadie—Hofstee approach is that neither ordinate nor abscissa represent scarchard variables: Archived from the original on The affinity constant is the inverse of the dissociation constant. I divided everything by 10E to get it all in M.
October Learn how and when to remove this template message. But don’t forget to label figs with what they are, scatchatd the units as well as numbers. Views Read Edit View history. From Wikipedia, the free encyclopedia.
Anyone know what I did wrong? BorekNov 25, Like I just thought it would be easier to scagchard the values without all the extra zeros.